Coding

Part:BBa_K4192022:Design

Designed by: Muhua Liu   Group: iGEM22_CAU_China   (2022-09-29)


FpOAR, oxalate efflux channel protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 158
    Illegal PstI site found at 301
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 158
    Illegal PstI site found at 301
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 158
    Illegal BglII site found at 1161
    Illegal XhoI site found at 973
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 158
    Illegal PstI site found at 301
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 158
    Illegal PstI site found at 301
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because it's a eukaryotic gene, although we have already tested it in some prokaryotic bacteria, the usage of it should still be careful.


Source

It is original from genomic sequence of Fomitopsis palustris strain TYP6137

References